A detailed representation of these measurement positions is shown in Figure 9. Imprint (d) = R (M c Ms) Equation 9 Figure 9: Schematic representation of the measurements required to calculate the final indentation depth.
This number of indentations is required for the final calculation of the modulus of elasticity. The values obtained with Equation 9 were used to calculate the modulus of elasticity with Equation 1, as described earlier in this article. Difference in Atomic Force Microscopy in the Preparation of a Polyacrylamide Gel for Atomic Force Microscopy to Dr. Yu Li Wang’s Protocol (Appendix) 10.2) A coverslip is required to apply the gel.
The glass is activated by applying various coatings described in the protocol. Due to the relative size of the AFM scan head, it is not possible to use a petri dish to store the polyacrylamide gel during the experiment. The active cover has a diameter of about three centimeters and serves as a mounting platform for samples. Unlike a petri dish, these sliders are smooth and do not interfere with the wet room or the AFM scan head. The caps themselves have yet to be activated, which is related to steps one to six of Dr. Wang’s protocol.
After determining the specific polyacrylamide concentration, about 200 ml is applied to the activated cover glass using a transfer pipette , and a smaller round or square cap is placed over the gel.
